![]() Furthermore, the peak with retention time 12.982 minutes with a similarity index of 20.757%, producing a mass spectrum with molecular ion peaks (M These spectra are expected to be methyl eugenol. GC-MS spectra gave two peaks with retention time 11.408 minutes with a similarity index of 79.243% with the molecular ion (M UV-Vis spectrum of the active isolates showed absorption at λ max 285 and 401 nm. Analysisof active isolates resulted the antioxidant activity (IC 50) of 100.00 ug / ml. The antioxidant activity (IC 50) active isolates were analyzed using spectrophotometry. The active compound was purified byPTLC and the purified active isolates was then confirmed by TLC. Active extract was partitioned with chloroform, methanol, distilled water and tested for antioxidant activity by DPPH 0.2% method. Antioxidant activity of extracts was evaluated with DPPH 0.2% method. Extracts obtained by evaporating the solvent wasbenzenon a rotary evaporator and were then re-macerated with methanol. The extraction of persimmon leaves was carried out by maceration method with wasbenzen. In the search for natural antioxidant compounds, have been studied isolation and identification of antioxidant compound of persimmon leaves (DiospyroskakiThunb.) using DPPH (2,2-diphenyl-1-pikrilhidrazil) method. One plant is efficacious as an antioxidant is the persimmon (Diospyros kakiThunb.) where is cultivated widely in East Asia, Spain and Indonesia. (3) Faculty of Pharmacy, Universitas Gadjah MadaĪntioxidant is a substance which in small concentrations can significantly inhibit or prevent the oxidation of the substrate. (2) Faculty of Pharmacy, Universitas Gadjah Mada (1) Faculty of Medicine and Nursing, University of Tanjungpura ![]() ![]() Isnindar Isnindar (1), Subagus Wahyuono (2), Erna Prawita Setyowati (3*) Kajian fitokimia menurut Harborne (1987) meliputi aneka ragam senyawa organik yang dibentuk dan disimpan oleh organisme, yaitu struktur kimianya, biosintesisnya. Ekstraksi menggunakan beragam pelarut yaitu n˗heksana, kloroform, metanol. Metode pengambilan sampel yang digunakan adalah metode purposif sampling. Sejalan dengan hal tersebut, Robinson (1991) menyatakan bahwa, metode ini diawali dengan mengisolasi kandungan senyawa metaboli t sekunder tersebut menggunakan metode ekstraksi pelarut seperti maserasi dan partisi. Menurut (Harborne, 1984) guna memperoleh informasi lebih awal mengenai kandungan kelompok senyawa metaboli t sekunder dapat diidentifikasi dengan metode fitokimia. ![]()
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